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microglial cultures derived from e13.5 mouse embryos  (Janvier Labs)

 
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    Janvier Labs microglial cultures derived from e13.5 mouse embryos
    <t>Microglial</t> cells were incubated with three different doses of JN403 (100 nM, 1 μM, and 10 μM) for 24 hours, followed by an assessment of cell viability using the MTT-assay. (A) JN403 treatment alone did not elicit cellular toxicity in microglial cells at concentrations up to 1 μM. non-significant (n.s). (B) Microglial cells were pre-treated with 100 nM or 1 μM of JN403 for 24 hours and additionally coincubated for another 24 hours with 1μM αSyn fragment 61-140 on the next day. αSyn induced cytotoxicity in microglial cells. Neither 100 nM nor 1 μM of JN403 treatment changed the cell viability. Data are presented as the mean ± standard error of the mean. Cell viability was measured for multiple independent experiments. **** p < 0.0001, significantly different from the control group.
    Microglial Cultures Derived From E13.5 Mouse Embryos, supplied by Janvier Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microglial+cultures+derived+from+e13%2E5+mouse+embryos/midbrain+tissue+of+e13+5+swiss+mouse+embryos/bio_rxiv__2020__04__04__996892-35-1-8
    Average 90 stars, based on 1 article reviews
    microglial cultures derived from e13.5 mouse embryos - by Bioz Stars, 2026-09
    90/100 stars

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    1) Product Images from "JN403, an alpha-7-nicotine-acetylcholine-receptor agonist, reduces alpha-synuclein induced inflammatory parameters of in vitro microglia but fails to attenuate the reduction of TH positive nigral neurons in a focal alpha-synuclein overexpression mouse model of Parkinson’s disease"

    Article Title: JN403, an alpha-7-nicotine-acetylcholine-receptor agonist, reduces alpha-synuclein induced inflammatory parameters of in vitro microglia but fails to attenuate the reduction of TH positive nigral neurons in a focal alpha-synuclein overexpression mouse model of Parkinson’s disease

    Journal: bioRxiv

    doi: 10.1101/2020.04.04.996892

    Microglial cells were incubated with three different doses of JN403 (100 nM, 1 μM, and 10 μM) for 24 hours, followed by an assessment of cell viability using the MTT-assay. (A) JN403 treatment alone did not elicit cellular toxicity in microglial cells at concentrations up to 1 μM. non-significant (n.s). (B) Microglial cells were pre-treated with 100 nM or 1 μM of JN403 for 24 hours and additionally coincubated for another 24 hours with 1μM αSyn fragment 61-140 on the next day. αSyn induced cytotoxicity in microglial cells. Neither 100 nM nor 1 μM of JN403 treatment changed the cell viability. Data are presented as the mean ± standard error of the mean. Cell viability was measured for multiple independent experiments. **** p < 0.0001, significantly different from the control group.
    Figure Legend Snippet: Microglial cells were incubated with three different doses of JN403 (100 nM, 1 μM, and 10 μM) for 24 hours, followed by an assessment of cell viability using the MTT-assay. (A) JN403 treatment alone did not elicit cellular toxicity in microglial cells at concentrations up to 1 μM. non-significant (n.s). (B) Microglial cells were pre-treated with 100 nM or 1 μM of JN403 for 24 hours and additionally coincubated for another 24 hours with 1μM αSyn fragment 61-140 on the next day. αSyn induced cytotoxicity in microglial cells. Neither 100 nM nor 1 μM of JN403 treatment changed the cell viability. Data are presented as the mean ± standard error of the mean. Cell viability was measured for multiple independent experiments. **** p < 0.0001, significantly different from the control group.

    Techniques Used: Incubation, MTT Assay, Control

    Inflammatory parameters (A) Nitric Oxide (NO) and (B) TNF-α were measured in JN403 pre-treated microglial cells for a baseline. (A) NO, and (B) TNF-α release increased with 1μM αSyn incubation, but both 100 nM and 1 μM of JN403 co-treatment significantly reduced NO and TNF-α release, * p < 0.05, ** p < 0.01.
    Figure Legend Snippet: Inflammatory parameters (A) Nitric Oxide (NO) and (B) TNF-α were measured in JN403 pre-treated microglial cells for a baseline. (A) NO, and (B) TNF-α release increased with 1μM αSyn incubation, but both 100 nM and 1 μM of JN403 co-treatment significantly reduced NO and TNF-α release, * p < 0.05, ** p < 0.01.

    Techniques Used: Incubation

    Iba1+ microglial cells were detected and the density analysis showed no differences between the rAAV groups and treatment groups. (A) Representative photomicrographs showing Iba1+ signals per group, TH (green) and Iba1 (magenta). No activated forms of microglia were found. Scale bar 200 μm. (B) The ratio of Iba1+ density of the injected side compared to the non-injected side showed no significant differences between the rAAVs and treatment, n=4, non-significant (n.s).
    Figure Legend Snippet: Iba1+ microglial cells were detected and the density analysis showed no differences between the rAAV groups and treatment groups. (A) Representative photomicrographs showing Iba1+ signals per group, TH (green) and Iba1 (magenta). No activated forms of microglia were found. Scale bar 200 μm. (B) The ratio of Iba1+ density of the injected side compared to the non-injected side showed no significant differences between the rAAVs and treatment, n=4, non-significant (n.s).

    Techniques Used: Injection

    Related Articles

    Derivative Assay:

    Article Title: JN403, an alpha-7-nicotine-acetylcholine-receptor agonist, reduces alpha-synuclein induced inflammatory parameters of in vitro microglia but fails to attenuate the reduction of TH positive nigral neurons in a focal alpha-synuclein overexpression mouse model of Parkinson’s disease
    Article Snippet: .. Briefly, microglial cultures derived from E13.5 mouse embryos (Janvier Breeding Center, France) were obtained by mild trypsinization as described previously. .. After 14 days, cultures were washed with Dulbecco’s modified Eagle’s medium/F-12 nutrient mixture (DMEM/F12; Invitrogen, Cergy-Pontoise, France) supplemented with 10% fetal calf serum (FCS) and then incubated with diluted DMEM/F12 (trypsin) 1:4 for 90 min at 37 °C until the astrocytic upper layer was detached.



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    microglial cultures derived from e13.5 mouse embryos  (Janvier Labs)
    90
    Janvier Labs microglial cultures derived from e13.5 mouse embryos
    <t>Microglial</t> cells were incubated with three different doses of JN403 (100 nM, 1 μM, and 10 μM) for 24 hours, followed by an assessment of cell viability using the MTT-assay. (A) JN403 treatment alone did not elicit cellular toxicity in microglial cells at concentrations up to 1 μM. non-significant (n.s). (B) Microglial cells were pre-treated with 100 nM or 1 μM of JN403 for 24 hours and additionally coincubated for another 24 hours with 1μM αSyn fragment 61-140 on the next day. αSyn induced cytotoxicity in microglial cells. Neither 100 nM nor 1 μM of JN403 treatment changed the cell viability. Data are presented as the mean ± standard error of the mean. Cell viability was measured for multiple independent experiments. **** p < 0.0001, significantly different from the control group.
    Microglial Cultures Derived From E13.5 Mouse Embryos, supplied by Janvier Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microglial+cultures+derived+from+e13%2E5+mouse+embryos/midbrain+tissue+of+e13+5+swiss+mouse+embryos/bio_rxiv__2020__04__04__996892-35-1-8
    Average 90 stars, based on 1 article reviews
    microglial cultures derived from e13.5 mouse embryos - by Bioz Stars, 2026-09
    90/100 stars
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    Microglial cells were incubated with three different doses of JN403 (100 nM, 1 μM, and 10 μM) for 24 hours, followed by an assessment of cell viability using the MTT-assay. (A) JN403 treatment alone did not elicit cellular toxicity in microglial cells at concentrations up to 1 μM. non-significant (n.s). (B) Microglial cells were pre-treated with 100 nM or 1 μM of JN403 for 24 hours and additionally coincubated for another 24 hours with 1μM αSyn fragment 61-140 on the next day. αSyn induced cytotoxicity in microglial cells. Neither 100 nM nor 1 μM of JN403 treatment changed the cell viability. Data are presented as the mean ± standard error of the mean. Cell viability was measured for multiple independent experiments. **** p < 0.0001, significantly different from the control group.

    Journal: bioRxiv

    Article Title: JN403, an alpha-7-nicotine-acetylcholine-receptor agonist, reduces alpha-synuclein induced inflammatory parameters of in vitro microglia but fails to attenuate the reduction of TH positive nigral neurons in a focal alpha-synuclein overexpression mouse model of Parkinson’s disease

    doi: 10.1101/2020.04.04.996892

    Figure Lengend Snippet: Microglial cells were incubated with three different doses of JN403 (100 nM, 1 μM, and 10 μM) for 24 hours, followed by an assessment of cell viability using the MTT-assay. (A) JN403 treatment alone did not elicit cellular toxicity in microglial cells at concentrations up to 1 μM. non-significant (n.s). (B) Microglial cells were pre-treated with 100 nM or 1 μM of JN403 for 24 hours and additionally coincubated for another 24 hours with 1μM αSyn fragment 61-140 on the next day. αSyn induced cytotoxicity in microglial cells. Neither 100 nM nor 1 μM of JN403 treatment changed the cell viability. Data are presented as the mean ± standard error of the mean. Cell viability was measured for multiple independent experiments. **** p < 0.0001, significantly different from the control group.

    Article Snippet: Briefly, microglial cultures derived from E13.5 mouse embryos (Janvier Breeding Center, France) were obtained by mild trypsinization as described previously.

    Techniques: Incubation, MTT Assay, Control

    Inflammatory parameters (A) Nitric Oxide (NO) and (B) TNF-α were measured in JN403 pre-treated microglial cells for a baseline. (A) NO, and (B) TNF-α release increased with 1μM αSyn incubation, but both 100 nM and 1 μM of JN403 co-treatment significantly reduced NO and TNF-α release, * p < 0.05, ** p < 0.01.

    Journal: bioRxiv

    Article Title: JN403, an alpha-7-nicotine-acetylcholine-receptor agonist, reduces alpha-synuclein induced inflammatory parameters of in vitro microglia but fails to attenuate the reduction of TH positive nigral neurons in a focal alpha-synuclein overexpression mouse model of Parkinson’s disease

    doi: 10.1101/2020.04.04.996892

    Figure Lengend Snippet: Inflammatory parameters (A) Nitric Oxide (NO) and (B) TNF-α were measured in JN403 pre-treated microglial cells for a baseline. (A) NO, and (B) TNF-α release increased with 1μM αSyn incubation, but both 100 nM and 1 μM of JN403 co-treatment significantly reduced NO and TNF-α release, * p < 0.05, ** p < 0.01.

    Article Snippet: Briefly, microglial cultures derived from E13.5 mouse embryos (Janvier Breeding Center, France) were obtained by mild trypsinization as described previously.

    Techniques: Incubation

    Iba1+ microglial cells were detected and the density analysis showed no differences between the rAAV groups and treatment groups. (A) Representative photomicrographs showing Iba1+ signals per group, TH (green) and Iba1 (magenta). No activated forms of microglia were found. Scale bar 200 μm. (B) The ratio of Iba1+ density of the injected side compared to the non-injected side showed no significant differences between the rAAVs and treatment, n=4, non-significant (n.s).

    Journal: bioRxiv

    Article Title: JN403, an alpha-7-nicotine-acetylcholine-receptor agonist, reduces alpha-synuclein induced inflammatory parameters of in vitro microglia but fails to attenuate the reduction of TH positive nigral neurons in a focal alpha-synuclein overexpression mouse model of Parkinson’s disease

    doi: 10.1101/2020.04.04.996892

    Figure Lengend Snippet: Iba1+ microglial cells were detected and the density analysis showed no differences between the rAAV groups and treatment groups. (A) Representative photomicrographs showing Iba1+ signals per group, TH (green) and Iba1 (magenta). No activated forms of microglia were found. Scale bar 200 μm. (B) The ratio of Iba1+ density of the injected side compared to the non-injected side showed no significant differences between the rAAVs and treatment, n=4, non-significant (n.s).

    Article Snippet: Briefly, microglial cultures derived from E13.5 mouse embryos (Janvier Breeding Center, France) were obtained by mild trypsinization as described previously.

    Techniques: Injection